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The Proceedings of the American Thoracic Society 2:537-540 (2005)
© 2005 The American Thoracic Society

Bioluminescence Imaging

Ruxana T. Sadikot and Timothy S. Blackwell

Division of Allergy, Pulmonary, and Critical Care Medicine, Department of Medicine, and Departments of Cell and Developmental Biology and Cancer Biology, Vanderbilt University School of Medicine; and Department of Veterans Affairs, Nashville, Tennessee

Correspondence and requests for reprints should be addressed to Timothy S. Blackwell, M.D., Vanderbilt University School of Medicine, T-1218 MCN, Nashville, TN 37232-2650. E-mail: timothy.blackwell{at}vanderbilt.edu

ABSTRACT

Bioluminescence refers to the process of visible light emission in living organisms. Bioluminescence imaging is a powerful methodology that has been developed over the last decade as a tool for molecular imaging of small laboratory animals, enabling the study of ongoing biological processes in vivo. This form of optical imaging is low cost and noninvasive and facilitates real-time analysis of disease processes at the molecular level in living organisms. In this article, we provide a brief introduction to bioluminescence imaging technology and discuss its applications in mouse models of lung inflammation/injury, bacterial pneumonia, and tumor growth and metastasis.

Key Words: nuclear factor kappa B • lung • endotoxin • pneumonia • cancer


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Proceedings of the American Thoracic Society 2005 2: 499-516. [Full Text]  



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