HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wurfel, M. M. Search for Related Content PubMed PubMed Citation Articles by Wurfel, M. M.

Microarray-based Analysis of Ventilator-induced Lung Injury

Division of Pulmonary and Critical Care Medicine, Harborview Medical Center, University of Washington, Seattle, Washington

Correspondence and requests for reprints should be addressed to Mark M. Wurfel, Harborview Medical Center, Research and Training Bldg, Rm 606, Box 359640, 300 9th Ave., Seattle, WA, 98104. E-mail: mwurfel{at}u.washington.edu

ABSTRACT

Acute respiratory distress syndrome (ARDS) and acute lung injury (ALI) are a frequent cause of intensive care unit admission, affecting over 200,000 patients in the United States each year. Mechanical ventilation is a life-saving intervention in the setting of ARDS and ALI, but clinical trials have demonstrated that mechanical ventilation with excessive tidal volumes plays a role in promoting and perpetuating lung injury and leads to excess mortality. This process has been labeled ventilator-induced lung injury (VILI), but the molecular mechanisms driving this process and its interactions with predisposing risk factors such as sepsis and chemical injury remain incompletely understood. Genome-wide measurements of gene expression using microarray technology represent a powerful tool to examine the pathophysiology of VILI. Several recent studies have used this approach to study VILI in isolation and associated with endotoxin instillation or saline lavage. These studies and others examining gene expression profiles in epithelial cells subjected to cyclic stretch have provided novel insights on the molecular mechanisms underlying VILI. This review will summarize these findings and discuss implications for future studies.

Key Words: microarray analysis • lung injury • mechanical ventilation • gene expression • genomics

This article has been cited by other articles:

				F.-X. Ble, C. Cannet, S. Zurbruegg, H. Karmouty-Quintana, R. Bergmann, N. Frossard, A. Trifilieff, and N. Beckmann Allergen-induced Lung Inflammation in Actively Sensitized Mice Assessed with MR Imaging Radiology, September 1, 2008; 248(3): 834 - 843. [Abstract] [Full Text] [PDF]

				P. E. Parsons Genetics, Iron, and ALI: An Intriguing Relationship Chest, June 1, 2008; 133(6): 1295 - 1296. [Full Text] [PDF]

				Z. Wang, D. Beach, L. Su, R. Zhai, and D. C. Christiani A Genome-Wide Expression Analysis in Blood Identifies Pre-Elafin as a Biomarker in ARDS Am. J. Respir. Cell Mol. Biol., June 1, 2008; 38(6): 724 - 732. [Abstract] [Full Text] [PDF]

				T. R. Martin Interactions between Mechanical and Biological Processes in Acute Lung Injury Proceedings of the ATS, April 15, 2008; 5(3): 291 - 296. [Abstract] [Full Text] [PDF]

				K. Hamanaka, M.-Y. Jian, D. S. Weber, D. F. Alvarez, M. I. Townsley, A. B. Al-Mehdi, J. A. King, W. Liedtke, and J. C. Parker TRPV4 initiates the acute calcium-dependent permeability increase during ventilator-induced lung injury in isolated mouse lungs Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L923 - L932. [Abstract] [Full Text] [PDF]

				J. U. Raj, C. Aliferis, R. M. Caprioli, A. W. Cowley Jr., P. F. Davies, M. W. Duncan, D. J. Erle, S. C. Erzurum, P. W. Finn, H. Ischiropoulos, et al. Genomics and proteomics of lung disease: conference summary Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L45 - L51. [Full Text] [PDF]