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A Proteomic Primer for the Clinician

Departments of Medicine, Biological Chemistry, and Biomedical Engineering, The Johns Hopkins University, Baltimore, Maryland

Correspondence and requests for reprints should be addressed to Yurong Guo, Ph.D., Johns Hopkins University Bayview Campus, 5200 Eastern Avenue, Mason F. Lord Building, Center tower, Room 609, Baltimore, MD 21224. E-mail: yguo7{at}jhmi.edu

ABSTRACT

Proteomics is a rapidly developing field and it opens new horizons in many research areas of life sciences. In the field of medicine, proteomics promises to accelerate the discovery of new drug targets and protein disease markers useful for in vitro diagnosis. In this article, we review the current proteomics technologies for biomarker discovery and validation, which include two-dimensional gel electrophoresis, one- and two-dimensional liquid chromatography, and proteomic microarrays. We will also review proteomic strategies for protein–protein interactions and identification of post-translational modifications. Selection of the more effective technology or combination of technologies is required to maximize the interpretation and utility of the data.

Key Words: 2D electrophoresis • multiple dimensional chromatography • post-translational modification • proteomic microarrays • proteomics

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