Proceedings of the American Thoracic Society
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The Proceedings of the American Thoracic Society 6:450-457 (2009)
© 2009 The American Thoracic Society
doi: 10.1513/pats.200902-010AW
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Well-defined Cationic Shell Crosslinked Nanoparticles for Efficient Delivery of DNA or Peptide Nucleic Acids

Ke Zhang1,2,*, Huafeng Fang1,*, Gang Shen1, John-Stephen A. Taylor1 and Karen L. Wooley1,2

1 Department of Chemistry, Washington University, St. Louis, Missouri; and 2 Department of Radiology, Washington University School of Medicine, St. Louis, Missouri

Correspondence and requests for reprints should be addressed to Karen L. Wooley, Ph.D., Washington University, Department of Chemistry, One Brookings Drive, CB1134, Saint Louis, MO 63130. E-mail: klwooley{at}wustl.edu

ABSTRACT

This mini-review highlights developments that have been made over the past year to advance the construction of well-defined nanoscale objects to serve as devices for cell transfection. Design of the nanoscale objects originated from biomimicry concepts, using histones as the model, to afford cationic shell crosslinked knedel-like (cSCK) nanoparticles. Packaging and delivery of plasmid DNA, oligonucleotides, and peptide nucleic acids were studied by dynamic light scattering, transmission electron microscopy, gel electrophoresis, biological activity assays, RT-PCR measurements, flow cytometry, and confocal fluorescence microscopy. With the demonstration of more efficient cell transfection in vitro than that achieved using commercially-available transfection agents, together with the other features offered by the robust nanostructural framework, work continues toward the application of these cSCKs for in vivo molecular recognition of genetic material, for imaging and therapy targeted specifically to pulmonary injury and disease.

Key Words: biomimicry • transfection • cationic nanoparticle • gene delivery


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Chair's Summary
Vladimir R. Muzykantov
Proceedings of the American Thoracic Society 2009 6: 398-402. [Full Text]  





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